I'm trying to understand how to analyse Immunohistochemistry (IHC). Could you explain the steps and key considerations involved in this process? I want to ensure accurate interpretation of the staining patterns and intensity.
5 answers
Sara
Fri Nov 01 2024
IHC samples can be visualized using various methods based on antibody detection.
CryptoChieftainGuard
Fri Nov 01 2024
One common method is light microscopy, which allows researchers to observe the samples under a microscope.
Margherita
Thu Oct 31 2024
Another option is fluorescence microscopy, which utilizes fluorescent dyes to highlight specific antigens in the sample.
Tommaso
Thu Oct 31 2024
When additional detail is required in fluorescence microscopy, confocal imaging systems can be employed.
Tommaso
Thu Oct 31 2024
These systems provide high-resolution images by focusing on a single plane within the sample.