Cryptocurrency Q&A
How to make floxed mice?
How to make floxed mice?
CryptoPioneer
Sun Sep 15 2024
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7 answers
1811
Could you please elaborate on the process of creating floxed mice? What specific steps are involved in administering the fluoroquinolone antibiotic to the animals? Are there any precautions or considerations that need to be taken into account during the process? Additionally, how does this treatment affect the mice's physiology and behavior, and what are the potential implications for studying the effects of fluoroquinolones in humans?
7 answers
Martina
Tue Sep 17 2024
The integration of the floxed allele and Cre recombinase technology offers unparalleled precision and control in genetic experiments. It enables scientists to study the function of specific genes in a living organism, without disrupting the overall genetic blueprint.
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SejongWisdomSeeker
Tue Sep 17 2024
In the realm of genetic engineering, a pivotal step involves the manipulation of specific gene sequences. One such technique entails the introduction of two short, mirrored sequences, colloquially known as LoxP sites, flanking a crucial segment of the target gene.
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Arianna
Tue Sep 17 2024
The strategic positioning of these LoxP sites creates a unique genetic configuration known as a "floxed" allele. This modified allele serves as a versatile platform for subsequent genetic modifications.
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ethan_carter_engineer
Tue Sep 17 2024
The floxed allele's significance lies in its compatibility with the Cre recombinase enzyme. Cre recombinase is a potent catalyst that triggers the recombination, or rearrangement, of genetic material at the LoxP sites.
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OliviaTaylor
Tue Sep 17 2024
By mating mice that harbor the floxed allele with those expressing Cre recombinase, researchers can precisely manipulate the genetic makeup of the offspring. This process allows for the conditional activation or inactivation of genes, depending on the experimental design.
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