I'm wondering about the purpose of SSC. I want to know what it's commonly used for and its applications in different fields.
6 answers
CryptoTamer
Sun Nov 10 2024
Saline-sodium citrate (SSC) buffer plays a crucial role in biochemistry and molecular biology.
Ilaria
Sun Nov 10 2024
It is widely utilized as a hybridization buffer in various experimental procedures.
Margherita
Sun Nov 10 2024
SSC buffer helps to regulate the stringency of washing steps, which is essential for ensuring the accuracy of results.
CryptoVanguard
Sat Nov 09 2024
In Southern blotting, SSC buffer is used to wash off unbound probes, allowing for the visualization of specific DNA sequences.
CryptoQueenBee
Sat Nov 09 2024
Similarly, in situ hybridization employs SSC buffer to control the hybridization and washing conditions, enabling the detection of RNA or DNA sequences within tissue samples.